wizard plus sv mini preps plasmid dna purification kit  (Promega)

Journal: Microorganisms

Article Title: Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

doi: 10.3390/microorganisms8081135

Figure Lengend Snippet: Plasmid profile of representative multidrug-resistant (MDR) APEC isolates. Agarose gel electrophoretogram (0.8% gel) of plasmid DNA of the MDR isolates: APEC strain DH80, DH69, DH106, RN42, NR45, RN 3(2), DH53, DH116, RN07, RN35, NR10 (from lane 3 to lane 14), E. coli V517 in lane 2 was used as the marker, and its plasmids of different sizes (kb) have been denoted in the figure. Lane 1 is molecular ladder. Plasmid bands at the same position was observed in APEC strain DH80, RN3(2) at the position between 3.9 kb; strain DH69, RN42 at the positions 3.0 kb, strain DH53, RN101 at the position of about 7.3 kb, and strain DH106, DH53, RN3(2) at the positions between 5.2 to 3.0 kb. Large plasmids at common positions were observed in strain DH80, DH106, DH116, RN101. Strain RN07, RN35, NR10 did not harbor any plasmid.

Article Snippet: Briefly, plasmid DNA of 45 randomly selected E. coli isolates was extracted using Wizard ® Plus SV Mini preps plasmid DNA Purification kit (Promega, USA) according to manufacturer’s instruction, and was electrophoresed in 0.8% agarose gel [ ].

Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Marker

Journal: bioRxiv

Article Title: Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

doi: 10.1101/2020.06.30.180257

Figure Lengend Snippet: Plasmid profile of representative isolates of the multidrug resistant (MDR) E. coli : Agarose gel electrophoretogram (0.8% gel) of plasmid DNA of the MDR isolates: E. coli strain DH80, DH69, DH106, RN42, NR45, RN 3(2), DH53, DH116, RN07, RN35, NR10 (from lane 3 to lane 14), E. coli V517 in lane 2 was used as the marker and its plasmids of different sizes (kb) have been denoted in the figure. Lane 1 is molecular ladders. Plasmid bands at the same position was observed in E. coli strain DH80, RN3(2) at the position between 3.9 kb; strain DH69, RN42 at the positions 3.0 kb, strain DH53, RN101 at the position of about 7.3 kb and strain DH106, DH53, RN3(2) at the positions between 5.2 kb to 3.0 kb. Large plasmids at common positions were observed in strain DH80, DH106, DH116, RN101. Strain RN07, RN35, NR10 din not harbored any plasmid.

Article Snippet: Briefly, plasmid DNA of 45 randomly selected E. coli isolates was extracted using Wizard® Plus SV Mini preps plasmid DNA Purification kit (Promega, USA) according to manufacturer’s instruction and was electrophoresed in 0.8% agarose gel ( ).

Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Marker